DYNAMIC LIGHT SCATTERING MACHINE HOW TO
DYNAMIC LIGHT SCATTERING MACHINE FULL

Now instead of a microsecond, jump forward a full second. In other words, they have a high correlation. Odds are good most particles haven’t moved around yet – so the light scattering hasn’t changed and the data from time zero and a microsecond later are about the same.

DYNAMIC LIGHT SCATTERING MACHINE HOW TO

Here’s how to think about analyzing light scattering data for DLS: pick a point in time – now jump forward a microsecond. Analyzing whether light intensity is changing fast or slow – that’s the secret sauce of DLS. Vice versa for larger ones because they are slower to move around. Since small particles zip around quickly, the intensity of light changes quickly. When the laser wavelength is much larger than the particles, you get equal amounts of light scattering in every direction – that’s why we use a 660 nm laser in our DLS systems.ĭLS can tell you a lot about the size of the particles in solution by measuring how rapidly that scattered light changes over time (Figure 1). Shine a laser on a solution of particles and you’ll get plenty of light scattering back out at you. Monoclonal antibodies & recombinant proteins.Ultrafiltration & Diafiltration (UF/DF).Differential Scanning Fluorimetry (DSF).The one of a kind silicone thickness and distribution analyzer Bouncer – The one of a kind silicone thickness and distribution analyzer.The most customizable automated workflow solution Big Kahuna – The most customizable automated workflow solution.The ultimate automated buffer exchange solution Big Tuna – The ultimate automated buffer exchange solution.

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